megfp c1 construct (Addgene inc)
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Addgene inc
megfp c1 construct
Megfp C1 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/megfp+c1+construct/mEGFP-C1+(Plasmid+%2354759)/10__1038_slash_s41467___026___78058___y-607-22-24
Average 94 stars, based on 82 article reviews
Megfp C1 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/megfp+c1+construct/mEGFP-C1+(Plasmid+%2354759)/10__1038_slash_s41467___026___78058___y-607-22-24
Average 94 stars, based on 82 article reviews
megfp c1 construct - by Bioz Stars,
2026-10
94/100 stars
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Amplification:Article Title: PARP10 condensation inhibits viral replication via targeting NAD+ homeostasis Article Snippet: Cells were treatedwith 500μMsodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300- 01 A), 1–30μM OUL-35 (R&D, 6344), 1μM OUL-232 (MCE, HY-148566), 5μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100μg/mL RNAse A (Takara, 740505) in PBS, 0.5μMLysoTracker (Thermo Fisher Scientific, L12492) for 30min at 37 °C, 1μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30min at 37 °C, 10μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1–2mM Nicotinamide riboside chloride (NR) (MCE, HY123033A) or 1μMFK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of Article Title: PARP10 condensation inhibits viral infection via targeting NAD + homeostasis Article Snippet: Cells were treated with 500 μM sodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300-01A) for 24 h, 10 μM Olaparib (MCE, HY-10162) or OUL-35 (R&D, 6344) for 5 h, VSV at MOI=1.5 for indicated times, SFV at MOI=0.5 for indicated times, 50 μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100 μg/mL RNAse A (Takara, 740505) in PBS, 0.5 μM LysoTracker (Thermo Fisher Scientific, L12492) for 30 min at 37 °C, 1 μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30 min at 37 °C, 10 μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1-2 mM Nicotinamide riboside chloride (NR) (MCE, HY-123033A) or 1 μM FK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of cDNA Library Assay:Article Title: PARP10 condensation inhibits viral replication via targeting NAD+ homeostasis Article Snippet: Cells were treatedwith 500μMsodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300- 01 A), 1–30μM OUL-35 (R&D, 6344), 1μM OUL-232 (MCE, HY-148566), 5μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100μg/mL RNAse A (Takara, 740505) in PBS, 0.5μMLysoTracker (Thermo Fisher Scientific, L12492) for 30min at 37 °C, 1μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30min at 37 °C, 10μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1–2mM Nicotinamide riboside chloride (NR) (MCE, HY123033A) or 1μMFK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of Article Title: PARP10 condensation inhibits viral infection via targeting NAD + homeostasis Article Snippet: Cells were treated with 500 μM sodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300-01A) for 24 h, 10 μM Olaparib (MCE, HY-10162) or OUL-35 (R&D, 6344) for 5 h, VSV at MOI=1.5 for indicated times, SFV at MOI=0.5 for indicated times, 50 μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100 μg/mL RNAse A (Takara, 740505) in PBS, 0.5 μM LysoTracker (Thermo Fisher Scientific, L12492) for 30 min at 37 °C, 1 μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30 min at 37 °C, 10 μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1-2 mM Nicotinamide riboside chloride (NR) (MCE, HY-123033A) or 1 μM FK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of Polymerase Chain Reaction:Article Title: PARP10 condensation inhibits viral replication via targeting NAD+ homeostasis Article Snippet: Cells were treatedwith 500μMsodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300- 01 A), 1–30μM OUL-35 (R&D, 6344), 1μM OUL-232 (MCE, HY-148566), 5μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100μg/mL RNAse A (Takara, 740505) in PBS, 0.5μMLysoTracker (Thermo Fisher Scientific, L12492) for 30min at 37 °C, 1μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30min at 37 °C, 10μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1–2mM Nicotinamide riboside chloride (NR) (MCE, HY123033A) or 1μMFK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of Article Title: PARP10 condensation inhibits viral infection via targeting NAD + homeostasis Article Snippet: Cells were treated with 500 μM sodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300-01A) for 24 h, 10 μM Olaparib (MCE, HY-10162) or OUL-35 (R&D, 6344) for 5 h, VSV at MOI=1.5 for indicated times, SFV at MOI=0.5 for indicated times, 50 μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100 μg/mL RNAse A (Takara, 740505) in PBS, 0.5 μM LysoTracker (Thermo Fisher Scientific, L12492) for 30 min at 37 °C, 1 μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30 min at 37 °C, 10 μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1-2 mM Nicotinamide riboside chloride (NR) (MCE, HY-123033A) or 1 μM FK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of Construct:Article Title: PARP10 condensation inhibits viral replication via targeting NAD+ homeostasis Article Snippet: Cells were treatedwith 500μMsodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300- 01 A), 1–30μM OUL-35 (R&D, 6344), 1μM OUL-232 (MCE, HY-148566), 5μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100μg/mL RNAse A (Takara, 740505) in PBS, 0.5μMLysoTracker (Thermo Fisher Scientific, L12492) for 30min at 37 °C, 1μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30min at 37 °C, 10μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1–2mM Nicotinamide riboside chloride (NR) (MCE, HY123033A) or 1μMFK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of Article Title: PARP10 condensation inhibits viral infection via targeting NAD + homeostasis Article Snippet: Cells were treated with 500 μM sodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300-01A) for 24 h, 10 μM Olaparib (MCE, HY-10162) or OUL-35 (R&D, 6344) for 5 h, VSV at MOI=1.5 for indicated times, SFV at MOI=0.5 for indicated times, 50 μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100 μg/mL RNAse A (Takara, 740505) in PBS, 0.5 μM LysoTracker (Thermo Fisher Scientific, L12492) for 30 min at 37 °C, 1 μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30 min at 37 °C, 10 μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1-2 mM Nicotinamide riboside chloride (NR) (MCE, HY-123033A) or 1 μM FK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of Cloning:Article Title: PARP10 condensation inhibits viral replication via targeting NAD+ homeostasis Article Snippet: Cells were treatedwith 500μMsodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300- 01 A), 1–30μM OUL-35 (R&D, 6344), 1μM OUL-232 (MCE, HY-148566), 5μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100μg/mL RNAse A (Takara, 740505) in PBS, 0.5μMLysoTracker (Thermo Fisher Scientific, L12492) for 30min at 37 °C, 1μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30min at 37 °C, 10μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1–2mM Nicotinamide riboside chloride (NR) (MCE, HY123033A) or 1μMFK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of Article Title: PARP10 condensation inhibits viral infection via targeting NAD + homeostasis Article Snippet: Cells were treated with 500 μM sodium arsenite (Merck, 1.06277.1003) for 1 h, 20 ng/mL human IFNβ (Peprotech, 300-02BC), IFNγ (Peprotech, 300-02), TNFα (Peprotech, 300-01A) for 24 h, 10 μM Olaparib (MCE, HY-10162) or OUL-35 (R&D, 6344) for 5 h, VSV at MOI=1.5 for indicated times, SFV at MOI=0.5 for indicated times, 50 μg/mL poly(I:C) LMW (Invivogen, tlrl-picw) for 5 h, 100 μg/mL RNAse A (Takara, 740505) in PBS, 0.5 μM LysoTracker (Thermo Fisher Scientific, L12492) for 30 min at 37 °C, 1 μM CellTracker CM-Dil Dye (Invitrogen, C7000) for 30 min at 37 °C, 10 μM BX795 (MCE, HY-10514) 3 h before and during viral infection, 1-2 mM Nicotinamide riboside chloride (NR) (MCE, HY-123033A) or 1 μM FK866 (MCE, HY-50876) 24 h before and during viral infection. .. DNA fragments encoding human PARP10 were amplified from U2OS cDNA library by PCR and ligated into the HindIII and BamHI sites of |